Stachys pilifera Benth. Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Rats through the Antioxidant Pathways

Background and Aim. Fibrosis of the lungs is an interstitial lung disease whose diagnosis treatment are not well-established. Stachys pilifera Benth. (Lamiaceae) has been proven to have antioxidant anti-inflammatory properties. This study was performed determine effect S. extract on bleomycin-induced pulmonary fibrosis in rats. Methods. In this experimental study, 35 male Wistar rats (120–180 g) were divided into five groups (n = 7) as follows: intratracheal instillation bleomycin (BLM, 7.5 IU/kg) administered group II. The third fourth received BLM plus hydroalcoholic (SPHE) (300 mg/kg/day, gavage). Vitamin E (500 gavage) given V addition BLM. After 14 days, animals euthanized assess biochemical parameters histopathology. Malondialdehyde (MDA), nitric oxide (NO), total thiol (TSH), glutathione (GSH) levels measured. addition, hydroxyproline (HYP) along with histological changes tissue also assessed. Results. MDA, NO, HYP elevations induced by toxicity significantly inhibited SPHE 600 mg/kg), Vit E. increased GSH TSH comparison group.HPLC analyses showed presence thymol (55.47 ng/mL) carvacrol (109.91 potential bioactive phenolic compounds. Conclusion. results suggest that alleviates development BLM-induced inhibiting proliferation fibroblasts mediated pathways. Other mechanisms underlying Effect need be clarified through further research.